Polymerase chain reaction for detection of measles virus in clinical samples

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Polymerase chain reaction for detection of measles virus in clinical samples.

A rapid and sensitive one-step reverse transcription polymerase chain reaction assay was developed to detect measles virus (MV) in nasal aspirates from patients with suspected MV infection. Oligonucleotide primers and probe were targeted to highly conserved regions of the matrix gene. Assay conditions were optimized to allow detection of as little as 1 PFU of an MV stock whose titer was known. ...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1993

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.31.5.1034-1039.1993